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ha tagged rictor  (Addgene inc)


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    Structured Review

    Addgene inc ha tagged rictor
    Ha Tagged Rictor, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myc+rictor/Rictor-Myc+(Plasmid+%23157931)/pm41787128-322-6-10
    Average 92 stars, based on 6 article reviews
    ha tagged rictor - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Over Expression:

    Article Title: Increased Levels of Rictor Prevent Mutant Huntingtin-Induced Neuronal Degeneration.
    Article Snippet: Rictor associates with mTOR to form the mTORC2 complex, which activity regulates neuronal function and survival.. Neurodegenerative diseases are characterized by the presence of neuronal dysfunction and cell death in specific brain regions such as for example Huntington’s disease (HD), which is characterized by the loss of striatal projection neurons leading to motor dysfunction.. Although HD is caused by the expression of mutant huntingtin, cell death occurs gradually suggesting that neurons have the capability to activate compensatory mechanisms to deal with neuronal dysfunction and later cell death.

    Transfection:

    Article Title: Increased Levels of Rictor Prevent Mutant Huntingtin-Induced Neuronal Degeneration.
    Article Snippet: Rictor associates with mTOR to form the mTORC2 complex, which activity regulates neuronal function and survival.. Neurodegenerative diseases are characterized by the presence of neuronal dysfunction and cell death in specific brain regions such as for example Huntington’s disease (HD), which is characterized by the loss of striatal projection neurons leading to motor dysfunction.. Although HD is caused by the expression of mutant huntingtin, cell death occurs gradually suggesting that neurons have the capability to activate compensatory mechanisms to deal with neuronal dysfunction and later cell death.

    Expressing:

    Article Title: Increased Levels of Rictor Prevent Mutant Huntingtin-Induced Neuronal Degeneration.
    Article Snippet: Rictor associates with mTOR to form the mTORC2 complex, which activity regulates neuronal function and survival.. Neurodegenerative diseases are characterized by the presence of neuronal dysfunction and cell death in specific brain regions such as for example Huntington’s disease (HD), which is characterized by the loss of striatal projection neurons leading to motor dysfunction.. Although HD is caused by the expression of mutant huntingtin, cell death occurs gradually suggesting that neurons have the capability to activate compensatory mechanisms to deal with neuronal dysfunction and later cell death.

    Article Title: mTORC2 Assembly Is Regulated by USP9X-Mediated Deubiquitination of RICTOR.
    Article Snippet: .. The following expression vectors were used: myc-RICTOR (#11367) (Sarbassov et al., 2004), myc-mTOR (#1861) (Jain et al., 2014), pCl-His-hUbi (#31815), N1-mSIN1.1-GFP (#72907), N1-mSIN1.2-GFP (#72908), N1-mSIN1.5-GFP (#72909) (Ebner et al., 2017), HA-ubiquitin (#18712) from Addgene (Young et al., 2011); pEGFP-C1-USP9X (MRC PPU Reagents and Services#DU10181): as a control empty pEGFP and pcDNA3.1-myc-His (Invitrogen) were used. ..

    Plasmid Preparation:

    Article Title: miR-192 inhibits the activation of hepatic stellate cells by targeting Rictor
    Article Snippet: LX-2 and HSC-T6 cells were cultured in Dulbecco’s Modified Eagle’s Medium (HyClone, USA) and supplemented with 10% fetal bovine serum (Gibco, Thermo Fisher Scientific, USA) and 1% penicillin/streptomycin (HyClone, USA) at 37°C with 5% CO 2 . .. miR-192-5p mimic, siRNA of Rictor, and negative controls were purchased from Guangzhou RiboBio, China. myc-Rictor corrected was a gift from David Sabatini (Addgene plasmid # 11367; http://n2t.net/addgene:11367 ; RRID: Addgene11367). .. Cells were transfected by using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.

    Article Title: Calcium/calmodulin-dependent protein kinase kinase 2 mediates pleiotropic effects of epidermal growth factor in cancer cells.
    Article Snippet: Water soluble PIP3 (C18) (#64930) was purchased from Cayman Chemical. .. AZD2014 (#A11303) was purchased from AdooQ. myc-Rictor was from Dr. David Sabatini, obtained from Addgene (plasmid #11367). p-Akt (T308) (#13038), p-Akt (S473) (#4060), panAkt (#4691), PDK1 (#13037), p-AMPK (T172) (#2525) and AMPK antibodies (#2603) were purchased from Cell Signaling Technology. .. CaMKK2 antibody for western blotting (#H00010645) was purchased from Novus and CaMKK2 antibody for immunofluorescence microscopy (#11549-1-AP) was purchased from Proteintech group.

    Control:

    Article Title: mTORC2 Assembly Is Regulated by USP9X-Mediated Deubiquitination of RICTOR.
    Article Snippet: .. The following expression vectors were used: myc-RICTOR (#11367) (Sarbassov et al., 2004), myc-mTOR (#1861) (Jain et al., 2014), pCl-His-hUbi (#31815), N1-mSIN1.1-GFP (#72907), N1-mSIN1.2-GFP (#72908), N1-mSIN1.5-GFP (#72909) (Ebner et al., 2017), HA-ubiquitin (#18712) from Addgene (Young et al., 2011); pEGFP-C1-USP9X (MRC PPU Reagents and Services#DU10181): as a control empty pEGFP and pcDNA3.1-myc-His (Invitrogen) were used. ..

    Construct:

    Article Title: MITF -MIR211 axis is a novel autophagy amplifier system during cellular stress
    Article Snippet: .. D.G. is a recipient of an EMBO Strategical Development and Installation Grant (EMBO-SDIG), Turkish Academy of Sciences (TUBA) GEBIP Award, IKU Prof. Dr. Onder Oztunali Science Award, TGC Sedat Simavi Health Sciences Award and Elginkan Foundation Technology Award. shRICTOR (Addgene, 1853) and MYC-RICTOR (Addgene, 11367) plasmids were kindly provided by David Sabatini. pEGFP-N1-TFEB (Addgene, 38119), pEGFP-N1-MITF-A (Addgene, 38132) constructs were kindly provided by Shawn Ferguson. .. RFP-LAMP1 (Addgene, 1817) was kindly provided by Walther Mothes.



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    Fig. 1 NUAK1 interacts with mTOR and <t>Rictor</t> but not with Raptor upon EGF stimulation. A Table shows total peptide count (P), the distributed spectra count (dS), and distributed normalized spectral abundance (dNSAF), observed for each identified protein in murine FLAG-NUAK1 WT and FLAG-NUAK1 KR44/71AA purifications (n = 3). NE, nuclear extract; CE, cytoplasmic extract. B Immunoblot (IB) of the Immunoprecipitation (IP) of human FLAG-NUAK1 WT and CoIP of endogenous mTOR, MYPT1 and exogenous Myc-Raptor in HEK293T cells. C IB of the IP of FLAG-NUAK1 WT and CoIP of endogenous mTOR, MYPT1 and exogenous Myc-Rictor in HEK293T cells. D IB of the IP of FLAG-NUAK1 WT and CoIP of endogenous mTOR, Rictor, Raptor and MYPT1 in MDA-MB-231 cells. E, F IB of the IP of FLAG-NUAK1 WT and CoIP of endogenous mTOR, Rictor, Raptor and MYPT1 from MDA-MB-231 (E) and U87 (F) cells serum-starved overnight before stimulation with EGF by 10 min. G IB of the IP of endogenous Rictor and CoIP of endogenous mTOR, and NUAK1 in MDA-MB-231 cells serum-starved overnight before stimulation with EGF by 10 min. H Proximity ligation assay (PLA) in MDA-MB-231 cells expressing HA-tagged NUAK1, FLAG-tagged NUAK1 or Empty vector (EV) (used as a negative control). Cells were serum-starved overnight and stimulated with EGF by 10 min (n = 3). Red dots indicate proximity of HA-NUAK1 with MYPT1 (Positive control), HA-NUAK1 with Rictor or FLAG-NUAK1 with mTOR. DAPI was used as a nuclear counterstain
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    Image Search Results


    Fig. 1 NUAK1 interacts with mTOR and Rictor but not with Raptor upon EGF stimulation. A Table shows total peptide count (P), the distributed spectra count (dS), and distributed normalized spectral abundance (dNSAF), observed for each identified protein in murine FLAG-NUAK1 WT and FLAG-NUAK1 KR44/71AA purifications (n = 3). NE, nuclear extract; CE, cytoplasmic extract. B Immunoblot (IB) of the Immunoprecipitation (IP) of human FLAG-NUAK1 WT and CoIP of endogenous mTOR, MYPT1 and exogenous Myc-Raptor in HEK293T cells. C IB of the IP of FLAG-NUAK1 WT and CoIP of endogenous mTOR, MYPT1 and exogenous Myc-Rictor in HEK293T cells. D IB of the IP of FLAG-NUAK1 WT and CoIP of endogenous mTOR, Rictor, Raptor and MYPT1 in MDA-MB-231 cells. E, F IB of the IP of FLAG-NUAK1 WT and CoIP of endogenous mTOR, Rictor, Raptor and MYPT1 from MDA-MB-231 (E) and U87 (F) cells serum-starved overnight before stimulation with EGF by 10 min. G IB of the IP of endogenous Rictor and CoIP of endogenous mTOR, and NUAK1 in MDA-MB-231 cells serum-starved overnight before stimulation with EGF by 10 min. H Proximity ligation assay (PLA) in MDA-MB-231 cells expressing HA-tagged NUAK1, FLAG-tagged NUAK1 or Empty vector (EV) (used as a negative control). Cells were serum-starved overnight and stimulated with EGF by 10 min (n = 3). Red dots indicate proximity of HA-NUAK1 with MYPT1 (Positive control), HA-NUAK1 with Rictor or FLAG-NUAK1 with mTOR. DAPI was used as a nuclear counterstain

    Journal: Cell & bioscience

    Article Title: NUAK1 coordinates growth factor-dependent activation of mTORC2 and Akt signaling.

    doi: 10.1186/s13578-023-01185-2

    Figure Lengend Snippet: Fig. 1 NUAK1 interacts with mTOR and Rictor but not with Raptor upon EGF stimulation. A Table shows total peptide count (P), the distributed spectra count (dS), and distributed normalized spectral abundance (dNSAF), observed for each identified protein in murine FLAG-NUAK1 WT and FLAG-NUAK1 KR44/71AA purifications (n = 3). NE, nuclear extract; CE, cytoplasmic extract. B Immunoblot (IB) of the Immunoprecipitation (IP) of human FLAG-NUAK1 WT and CoIP of endogenous mTOR, MYPT1 and exogenous Myc-Raptor in HEK293T cells. C IB of the IP of FLAG-NUAK1 WT and CoIP of endogenous mTOR, MYPT1 and exogenous Myc-Rictor in HEK293T cells. D IB of the IP of FLAG-NUAK1 WT and CoIP of endogenous mTOR, Rictor, Raptor and MYPT1 in MDA-MB-231 cells. E, F IB of the IP of FLAG-NUAK1 WT and CoIP of endogenous mTOR, Rictor, Raptor and MYPT1 from MDA-MB-231 (E) and U87 (F) cells serum-starved overnight before stimulation with EGF by 10 min. G IB of the IP of endogenous Rictor and CoIP of endogenous mTOR, and NUAK1 in MDA-MB-231 cells serum-starved overnight before stimulation with EGF by 10 min. H Proximity ligation assay (PLA) in MDA-MB-231 cells expressing HA-tagged NUAK1, FLAG-tagged NUAK1 or Empty vector (EV) (used as a negative control). Cells were serum-starved overnight and stimulated with EGF by 10 min (n = 3). Red dots indicate proximity of HA-NUAK1 with MYPT1 (Positive control), HA-NUAK1 with Rictor or FLAG-NUAK1 with mTOR. DAPI was used as a nuclear counterstain

    Article Snippet: To generate pCW57 FLAG-hNUAK1 WT, hNUAK1 WT was amplified from pCMV FLAG-hNUAK1 and subcloned into pCW57-MCS1-2 A-MCS2 using NheI and AgeI restriction sites. pCMV FLAG-hNUAK1 K84A was generated by subcloning of FLAG-hNUAK1 K84A from pBABE FLAG-hNUAK1 K84A using EcoRI restriction sites. pBABE FLAG-hNUAK1 K84A was previously generated by site direct mutagenesis using the following primers: hNUAK1_K84A_F: GGC CGA GTG GTT GCT ATA GCC TCC ATT CGT AAGG, hNUAK1_K84A_R: CCT TAC GAA TGG AGG CTA TAG CAA CCA CTC GGCC. pCMV FLAG-NUAK1 K84A mutation was confirmed by sequencing (Additional file 1: Fig. S6). pCW57MCS1-2 A-MCS2 (#71782), FLAG FOXO3a (#8360), pCMV dR8.2 (#8360), pCMV VSVG (#8454), pLKO scramble (#1864), pLKO shRictor (#1853), Lamp1-YFP (#2532), Lamp1-RFP (#1817), EGFR-GFP (#32751), pRK5 Myc Rictor (#11367), pRK5 Myc Raptor (#1859), and mRFP-Rab5 (#14437) from Addgene. pCMV6 Myr-Akt1HA was kindly provided by Dr. Philip Tsichlis, The Ohio State University, Rab7-GFP by Dr. Julio Tapia, Universidad de Chile, Chile, and pINDUCER10 shNUAK1 #1 and shNUAK1 #2 by Drs. Giacomo Cossa and Martin Eilers, University of Würzburg, Germany.

    Techniques: Western Blot, Immunoprecipitation, Proximity Ligation Assay, Expressing, Plasmid Preparation, Negative Control, Positive Control